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KMID : 0894520170210040371
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Development & Reproduction
2017 Volume.21 No. 4 p.371 ~ p.378
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Expression Analysis of Interferon-Stimulated Gene 15 in the Rock Bream Oplegnathus fasciatus against Rock Bream Iridovirus (RSIV) Challenge
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Kim Kyung-Hee
Yang In-Jung
Kim Woo-Jin
Park Choul-Ji
Park Jong-Won
Noh Gyeong-Eon
Lee Seung-Hyung
Lee Young-Mee
Hwang Hyung-Kyu
Kim Hyun-Chul
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Abstract
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Interferon-stimulated gene 15 (ISG15) is known to interfere with viral replication and infection by limiting the viral infection of cells. Interferon-stimulated gene 15 (ISG15) interferes with viral replication and infectivity by limiting viral infection in cells. It also plays an important role in the immune response. In this study, tissue-specific expression of ISG15 in healthy rock bream samples and spatial and temporal expression analysis of rock bream ISG15 (RbISG15) were performed following rock bream iridovirus (RSIV) infection. RbISG15 expression was significantly higher in the eye, gill, intestine, kid-ney, liver, muscle, spleen, and stomach, but low in the brain. There were particularly high levels of expression in the liver and muscle. RbISG15 expression was also examined in several tissues and at various times following RSIV infection. ISG15 ex-pression increased within 3 h in the whole body and decreased at 24 h after infection. In addition, temporal expression of sev-eral tissues following RSIV infection showed a similar pattern in the muscle, kidney, and spleen, increasing at 3 h and de-creasing at 72 h. These results suggest that ISG15 plays an important role in the immune response of rock bream. Overall, this study characterizes the response of RbISG15 following RSIV infection.
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KEYWORD
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Rock bream, Oplegnathus fasciatus, Gene expression, Rock bream iridovirus (RSIV), IFN-stimulated genes (ISGs)
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